A Chemoinformatics Approach for Enantioseparation Mechanism of Amino Acids by Micellar Electrokinetic Chromatography Using Bile Acids

Quantitation of chiral amino acids from microalgae by micellar electrokinetic chromatography and laser induced fluorescence detection

Running Title: Chiral analysis of microalgae amino acids by MEKC-LIF ABSTRACT In this work, a chiral and a non-chiral micellar electrokinetic chromatography with laser-induced fluorescence detection (MEKC-LIF) have been combined to identify and quantify a group of D-and L-amino acids (D/L-aa) in different microalgae samples. The combination of the non-chiral and chiral-MEKC-LIF methods made eas...

Precolumn diastereomerization and micellar electrokinetic chromatography on a plastic microchip: rapid chiral analysis of amino acids.

Precolumn derivatization and chiral separation of DL-amino acids based on diastereomerization have been performed on an integrated poly(dimethylsiloxane) microchip. Diastereomeric derivatives were formed in a microfabricated precolumn reactor by the reaction of amino acid enantiomers with o-phthaldialdehyde/2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranose (OPA/TATG), and separated by MEKC in ...

Chiral micellar electrokinetic chromatography-laser induced fluorescence of amino acids in foods: analysis of vinegars

Sensitive micellar electrokinetic chromatography-laser-induced fluorescence method to analyze chiral amino acids in orange juices.

In this work a new method to detect the existence of chiral amino acids in orange juice is presented. The method employs beta-cyclodextrins and micellar electrokinetic chromatography with laser-induced fluorescence (MEKC-LIF) to separate and detect L- and D-amino acids (L-aa and D-aa) previously derivatized with fluorescein isothiocianate (FITC). A systematic optimization of the chiral-MEKC con...

Enantioseparation of Penconazole, Myclobutanil and Triadimefon by Cyclodextrin-Modified Micellar Electrokinetic Chromatography

Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated with different HP-γ-CD concentrations as chiral selector. The three triazole fungicides have an asymmetrically substituted carbon atom respectively, and they all contain...